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PHO1 / Anti-Phosphate transporter PHO1 antibody AT3G23430.

Catalog Number: PHY0730S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT3G23430.

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PHO1 (At3g23430) is a protein with the mainly hydrophilic N-terminal and the C-terminal containing membrane-spanning domains and it has been proposed to function as a Pi transporter. It has been proved that PHO2 (AT2G33770) modulates the degradation of PHO1 in the endomembranes to maintain Pi homeostasis in plants. Arabidopsis contains 11 PHO proteins: PHO1 (AT3G23430), PHO1;H1 (AT1G68740), PHO1;H2 (AT2G03260), PHO1;H3 (AT1G14040), PHO1;H4 (AT4G25350), PHO1;H5 (AT2G03240), PHO1;H6 (AT2G03250), PHO1;H7 (AT1G26730), PHO1;H8 (AT1G35350), PHO1;H9 (AT3G29060), PHO1;H10 (AT1G69480).

  Synonyms:

PHO1, ARABIDOPSIS PHOSPHATE 1, ATPHO1, PHOSPHATE 1

  Immunogen:

KLH-conjugated synthetic peptide of PHO1 derived from Arabidopsis thaliana AT3G23430.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Applications: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

91 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 20 μg total protein from Arabidopsis thaliana leaf. 
Lane 2: 40 μg total protein from Arabidopsis thaliana leaf. 
Lane 3: 80 μg total protein from Arabidopsis thaliana leaf. 
Electrophoresis: 10% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.