PHO1 (At3g23430) is a protein with the mainly hydrophilic N-terminal and the C-terminal containing membrane-spanning domains and it has been proposed to function as a Pi transporter. It has been proved that PHO2 (AT2G33770) modulates the degradation of PHO1 in the endomembranes to maintain Pi homeostasis in plants. Arabidopsis contains 11 PHO proteins: PHO1 (AT3G23430), PHO1;H1 (AT1G68740), PHO1;H2 (AT2G03260), PHO1;H3 (AT1G14040), PHO1;H4 (AT4G25350), PHO1;H5 (AT2G03240), PHO1;H6 (AT2G03250), PHO1;H7 (AT1G26730), PHO1;H8 (AT1G35350), PHO1;H9 (AT3G29060), PHO1;H10 (AT1G69480).
KLH-conjugated synthetic peptide of PHO1 derived fromArabidopsis thalianaAT3G23430.
Serum Peptide affinity form antibody available upon request at email@example.com.
Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
For more species homologues information, please contact tech support at firstname.lastname@example.org.
Lane 1: 20 μg total protein fromArabidopsis thalianaleaf. Lane 2: 40 μg total protein fromArabidopsis thalianaleaf. Lane 3: 80 μg total protein fromArabidopsis thalianaleaf. Electrophoresis:10% SDS-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:1000 dilution overnight at 4℃. Secondary antibody:1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.