Transmembrane nine (TM9) proteins are localized in the secretory pathway of eukaryotic cells and are involved in cell adhesion and phagocytosis.
EMP12, ATTMN1, ENDOMEMBRANE PROTEIN 12, TMN1, TRANSMEMBRANE NINE 1
KLH-conjugated synthetic peptide of EMP12 derived fromArabidopsis thalianaAT1G10950.
Serum Peptide affinity form antibody available upon request at firstname.lastname@example.org.
Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
67 / 50 kDa
For more species homologues information, please contact tech support at email@example.com.
Lane 1: 20 μg total protein fromArabidopsis thalianaleaf. Lane 2: 40 μg total protein fromArabidopsis thalianaleaf. Lane 3: 80 μg total protein fromArabidopsis thalianaleaf. Electrophoresis:12% SDS-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:1000 dilution overnight at 4℃. Secondary antibody:1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.