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SNX2a / Anti-Sorting nexin 2A antibody AT5G58440

Catalog Number: PHY0935S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT5G58440

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

The Arabidopsis sorting nexins SNX1 (AT5G06140), SNX2a (AT5G58440) and SNX2b (AT5G07120) possess a PX domain and two coiled-coil regions constituting BAR domains at the C-terminus. Inhibition of retromer function in vivo by expression of SNX1 or SNX2a mutants as well as transient RNAi knockdown of all sorting nexins led to accumulation of the VSR BP80 at the TGN.

  Synonyms:

SNX2a

  Immunogen:

KLH-conjugated synthetic peptide of SNX2a derived from Arabidopsis thaliana AT5G58440.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Applications: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

18 / 12 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 3 μg stromal protein from Arabidopsis thaliana leaf. 
Lane 2: 6 μg stromal protein from Arabidopsis thaliana leaf. 
Lane 3: 12 μg stromal protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.