网站首页    代理品牌    50011 HDAC11, GST-tag

50011 HDAC11, GST-tag

Catalog #50011

Size: 50 µg
Description
Human HDAC11 , full length, GenBank Accession No. NM_024827, with N-terminal GST-tag, MW= 66 kDa, expressed in baculovirus expression system.
Species
Human
 
Host Species
Sf9 insect cells
 
MW
66 kDa
 
Genbank #
NM_024827
 
Tag(s)
N-terminal GST-tag
 
a.a.
full length
 
UniProt #
Q96DB2
 
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
 
Stability
>6 months at –80°C
 
Storage/Stability

>6 months at -80°C.

 
Shipping Temperature
-80
 
Synonym(s)
histone deacetylase 11, HDAC-11
 
Specific Activity
≥3.7 pmol/min/µg.
 
Assay Conditions
25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC class 2a substrate (Catalog #50040), and HDAC11 (15.6 - 2000 ng). Incubation condition: 35 min at 37°C, followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.
 
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol
 
Format
Aqueous buffer solution
 
References

Strausberg, R.L. et al., Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002).

Application Reference(s):
1. Reassessing the effects of histone deacetylase inhibitors on hippocampal memory and cognitive aging (2014)

 
Warnings
Avoid freeze/thaw cycles.
 
Scientific Category
Deacetylase