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ZKT抗体

ZKT是包含PDZ,K-box和TPR基训的新型植物蛋白家族的成员之一,受伤后ZKT的mRNA水平下降,ZKT蛋白可能是在伤口反应中受磷酸化调节的分子衔接子。
Catalog Number: PHY2644S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT1G55480

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

ZKT is a member of a novel plant protein family containing a PDZ, a K-box, and a TPR motif. The level of ZKT mRNA decreased after wounding. ZKT protein may act as a molecular adaptor regulated by phosphorylation in wound responses.

  Synonyms:

ZKT, PROTEIN CONTAINING PDZ DOMAIN, A K-BOX DOMAIN, AND A TPR REGION, MET1

  Immunogen:

KLH-conjugated synthetic peptide of ZKT derived from Arabidopsis thaliana AT1G55480.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:5000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

37 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napusBrassica rapa, and 80-99% homologues with the sequence in Sorghum bicolorZea maysOryza sativa Japonica GroupSetaria viridisHordeum vulgare.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 4 μl total chloroplast protein from Arabidopsis thaliana leaf.
Lane 2: 10 μg stromal protein from Arabidopsis thaliana leaf.
Lane 3: Thylakoid membrane protein from Arabidopsis thaliana leaf containing 2.5 μg of chlorophyll.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.