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Cat # 3031 Coelenterazine-SOL in vivo

Cat # 3031 Coelenterazine-SOL in vivo
Native Coelenterazine formulated to be water-soluble and safe for in vivo imaging applications with Gaussia Luciferase.
 
Coelenterazine is often dissolved in Propyleneglycol (PG) in combination with other alcohols for in vivo imaging studies. Large intravenous doses of PG given over a short period of time can be toxic, including  hyperosmolality, increased anion gap metabolic acidosis (due to lactic acidosis), acute kidney injury, and sepsis-like syndrome.

This can be avoided using water-soluble Coelenterazine. It is formulated to be isosmotic and easily secreted by the kidneys. Thus save for repeated i.v. injections. Water-soluble Coelenterazine can be injected up to a 500 µg/100 µl concentration, achieving very high Coelenterazine levels within the body and leading to high Gaussia luciferase signals.

 

Content:

native Coelenterazine + non-toxic additives for water-solublity

Packaging size:

500 ug CTZ / vial in a freeze-dried form

Storage:

 

sealed vials can be stored at:
-80°C for long-term storage
-20°C for short-term storage    Store dry and dark!

Application:

just add sterile water, vortex and use up to 500 ug CTZ in a single injection 

Application example:




















 

              

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

A detailed describtion of water soluble Coelenterazine can be found in:

 

A Water-Soluble Coelenterazine for Sensitive In Vivo Imaging of Coelenterate Luciferases. Danielle Morse and Bakhos A Tannous
Molecular Therapy 20, 692-693 (April 2012) | doi:10.1038/mt.2012.38

 

NEW! Researchers at Emory Medical School were successfully using NanoLights water-soluble CTZ via intranasal administration. More details can be found here:

Optochemogenetic Stimulation of Transplanted iPS-NPCs Enhances Neuronal Repair and Functional Recovery after Ischemic Stroke. Yu et al.
J Neurosci. 39(33):6571-6594, 2019 Aug 14 | PMID: 31263065



Controls:

 

 

 

 

 

 

 

We offer the solubilization vehicle without the Coelenterazine as injection control. Any side effects due to the injection process can be easily monitored, especially in sensitve applications like bioluminescent-driven Optogenetics. One vial of the control (Cat. #3031C) contains the same amount of solubilizer as one vial of Cat.#3031. The control will be dissolved in sterile water to create an iso-osmotic solution ready to inject.

To order control: please select '10 mgs + 10 vials control' under product options. This includes 20 vials of 500ug water soluble CTZ and 10 vials of control.
Please inquire if larger quantities of Cat.#3031C are needed.

Reference: Luminopsins integrate opto- and chemogenetics by using physical and biological light sources for opsin activation. Berglund K et al.
Proc Natl Acad Sci U S A. 113(3): E358-67 (January 2016) 
doi:10.1073/pnas.1510899113

Manual for CTZ-SOL (in vivo)      MSDS

Advantages of water-soluble Coelenterazine:

  • non-toxic, iso-osmotic, Propylene-Glycol free, Alcohol free
  • long shelf-life, sealed under vacuum
  • dissolves fast in sterile water
  • low viscosity - ideal for injections
  • consistent quality due to small aliquot sizes (10mgs will be shipped as 20x 500ug)

 Shipping: via FedEx or UPS at RT, overnight or 2nd delivery. Store at -80°C.