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CAS / Anti-Calcium sensing receptor, chloroplastic antibody

CAS是一种叶绿体定位蛋白,调节胞质Ca2+浓度,是调控气孔对外界Ca2+浓度升高的关键蛋白。针对CAS蛋白,PHYTOAB公司研发了相应的抗体PHY1740S,PHY1741S.
Catalog Number: PHY1740S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT5G23060

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

CAS is a chloroplast-localized protein that modulates cytoplasmic Ca2+ concentration and is crucial for proper stomatal regulation in response to elevations of external Ca2+.

  Synonyms:

CAS, CALCIUM SENSING RECEPTOR

  Immunogen:

Recombinant protein of CAS (1-185aa) derived from Arabidopsis thaliana AT5G23060.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum

  Reconstitution:  

Reconstitution with 150µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:30000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

41 / 35 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: Thylakoid membrane protein from Arabidopsis thaliana leaf containing 1 μg of chlorophyll.
Lane 2: 4 μl total chloroplast protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:30000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.