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VAB1/2/3 / Anti-V-type proton ATPase subunit B1/2/3 antibody

拟南芥V-ATP酶具有3个B型亚基(VAB1、VAB2和VAB3),它们具有97.27%的序列同源性,并都具有两个潜在的肌动蛋白结合位点,这些表明VABs在肌动蛋白细胞骨架重塑和植物细胞发育中可能具有重要作用。
针对VAB1/2/3蛋白,我公司研发了相应的抗体PHY2228S。
Catalog Number: PHY2228S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT1G76030, AT4G38510, AT1G20260

View FileData Sheet-PHY2228S

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Arabidopsis V-ATPase has three B subunits (AtVAB1, AtVAB2, and AtVAB3), which share 97.27% sequence identity and have two potential actin-binding sites, indicating that these AtVABs may have crucial functions in actin cytoskeleton remodeling and plant cell development.

  Synonyms:

VAB1/2/3, ATVAB1/2/3, V-ATPASE B SUBUNIT 1/2/3

  Immunogen:

KLH-conjugated synthetic peptide of VAB1/2/3 derived from Arabidopsis thaliana AT1G76030, AT4G38510, AT1G20260.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150 µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

54 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Hordeum vulgareGossypium raimondiiOryza sativa Japonica GroupZea maysVitis viniferaSorghum bicolorPopulus trichocarpaMedicago truncatulaGlycine maxBrassica rapaSetaria viridisCucumis sativusBrassica napusTriticum aestivumSpinacia oleracea, and 80-99% homologues with the sequence in Nicotiana tabacumSolanum lycopersicumSolanum tuberosumPhyscomitrella patens.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

20 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 12% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.