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蓝细菌CmpB抗体

CmpB蛋白是转运蛋白复合物CmpABCD的一员,该蛋白复合物参与碳酸氢盐的转运,只有低浓度CO2的环境能够诱导产生该蛋白复合物。
针对CmpB蛋白,我公司开发了相应的抗体PHY5223A。
Catalog Number: PHY5223A
Size: 150 µg
Confirmed reactivity:Synechocystis sp. PCC 6803
Database Access Number: slr0041  Q55461

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

cmpB is a part of the ABC transporter complex CmpABCD involved in bicarbonate transport. It is probably responsible for the translocation of the substrate across the membrane.

  Synonyms:

cmpB

  Immunogen:

KLH-conjugated synthetic peptide of cmpB derived from Synechocystis sp. PCC 6803 (slr0041).

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01 M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

31 / 35 kDa

  Confirmed Reactivity: 

Synechocystis sp. PCC 6803

  Predicted Reactivity: 

For homologues with other species especially algae, please contact tech support at tech@phytoab.com.

 

Application Example

10 μg whole-cell lysate protein from WT of Synechocystis sp. PCC 6803.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1.5 h.
Blocking: 5% skim milk at RT or 4℃ for 2 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.