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富含甘氨酸RNA结合蛋白GRP8抗体

GRP8 是富含甘氨酸的蛋白,其N端有RNA结合结构域。拟南芥中,RNA结合蛋白GRP7和GRP8在转录后水平上进行自身负向调控,并相互交叉调控,形成具有双稳态波动开关的分子振荡器,揭示了拟南芥生物钟的分子机制。

针对GRP8蛋白,我公司开发了相应的抗体PHY2458S。
Catalog Number: PHY2458S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT4G39260

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

RBG8 is a glycine-rich protein with RNA binding domain at the N-terminus. It plays a role in RNA transcription or processing during stress.

  Synonyms:

GRP8, ATGRP8, CCR1, COLD, CIRCADIAN RHYTHM, AND RNA BINDING 1, GLYCINE-RICH PROTEIN 8, GLYCINE-RICH RNA-BINDING PROTEIN 8, GR-RBP8, RBGA6, RNA-BINDING GLYCINE-RICH PROTEIN A6

  Immunogen:

KLH-conjugated synthetic peptide of GRP8 derived from Arabidopsis thaliana AT4G39260.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150 µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:5000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

17 / 18 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

6 μg nuclear protein from Arabidopsis thaliana.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.