5PRIME HotMasterMix - 1000R
5PRIME HotMasterMix1000 x 50 μL rxns (20 x 1 mL)Order (2200410)
Features & Benefits
- Optimal results – highly specific amplification
- Minimal handling – ready-to-use mastermix format
- Convenience – storage at 4°C eliminates freeze-thaw cycles
5PRIME HotMasterMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
The 5PRIME HotMasterMix is a ready-to-use reagent mix that offers high reproducibility when processing large numbers of samples. The HotMaster Taq DNA Polymerase, an integral component of the master mix, is designed to reduce or eliminate any non-specific products that may result from mispriming during PCR.
The 5PRIME HotMasterMix (2.5x) is a ready-to-use PCR mix. Only primers and template need to be added to the 2.5x concentrate, thus decreasing the number of time-consuming pipetting steps. This format not only reduces the likelihood of errors and the risk of contamination, but it also increases precision and sample throughput.
The 5PRIME HotMasterMix also contains the self-adjusting Mg2+ buffer technology. This formulation adjusts the Mg2+ concentration automatically, eliminating the need for optimizing this critical component. The MasterMix does not need to be stored frozen, eliminating the time-consuming thawing process and the resulting reduction in performance.
The 5PRIME HotMasterMix is a 2.5-fold concentrate, resulting in the following final concentrations for a 50 μl PCR reaction: 1 U Taq DNA Polymerase, 45 mM Cl, 2.5 mM Mg2+, 200 μM of each dNTP.
Storage & Handling