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叶绿体PSA3抗体

光合系统I(PSI)是一种大型的蛋白色素复合体,位于蓝细菌,植物和藻类的类囊体膜上,PSI的结构组分已经被深入的解析。核基因编码的光合系统I组装因子3(PSA3)能够在翻译后水平上调控PSI的稳定组装,PSI的Psac亚基极大可能是PSA3和PYG7的首要作用对象。针对PSA3蛋白,我公司开发了相应的抗体PHY2204
Catalog Number: PHY2204
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT3G55250

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Photosystem I (PSI) is a large protein-pigment complex located in the thylakoid membrane in cyanobacteria, plants, and algae. Photosystem I Assembly 3 (PSA3) is conserved among green photosynthetic eukaryotes and is required for PSI accumulation. PSA3 maybe cooperate with PYG7 to promote the stable assembly of PSI, and that the PsaC subunit is likely to be the primary target of their action.

  Synonyms:

PSA3, PDE329, PHOTOSYSTEM I ASSEMBLY 3, PIGMENT DEFECTIVE 329

  Immunogen:

Recombinant protein of PSA3 derived from Arabidopsis thaliana AT3G55250.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01 M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Applications: 

Western Blot (1:1000-1:5000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

31 / 25 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

20 μg total chloroplast protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.