赤霉素受体GID1A抗体

  Description:

Rabbit polyclonal antibody

  Background:

GID1A is a gibberellin (GA) receptor ortholog of the rice GA receptor gene (OsGID1). It has GA-binding activity, showing higher affinity to GA4. It interacts with DELLA proteins in vivo in the presence of GA4. Arabidopsis has three gibberellin receptors, GIBBERELLIN INSENSITIVE DWARF1 A (GID1A, AT3G05120), GID1B (AT3G63010) and GID1C (AT5G27320).

  Synonyms:

GID1A, ATGID1A, GA INSENSITIVE DWARF1A

  Immunogen:

KLH-conjugated synthetic peptide derived from N-terminal of GID1A protein in Arabidopsis thaliana AT3G05120.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

39 / 40 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

For more species homologues information, please contact tech support at tech@phytoab.com.

Lane 1: 40 μg total protein from Arabidopsis thaliana leaf.
Lane 2: 6 μg nuclear protein from Arabidopsis thaliana .
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.