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SNRK2.4/10 / Anti-Serine/threonine-protein kinase SRK2A/B antibody

Catalog Number: PHY0741A
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT1G10940, AT1G60940

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

SRK2A/B is a member of SNF1-related protein kinases (SnRK2) whose activity is activated by ionic (salt) and non-ionic (mannitol) osmotic stress.

  Synonyms:

SNRK2.4/10, SNF1-RELATED PROTEIN KINASE 2.4/10, SNRK2-4/10, SRK2A/B, SUCROSE NONFERMENTING 1-RELATED PROTEIN KINASE 2-4/10

  Immunogen:

KLH-conjugated synthetic peptide of SnRK2.4/10 derived from Arabidopsis thaliana AT1G10940, AT1G60940.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Applications: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

42 kDa (AT1G10940), 41 kDa (AT1G60940)

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica  rapaGlycine maxGossypium raimondiiNicotiana tabacum, and 80-99% homologues with the sequence in Brassica napusSolanum lycopersicumSolanum tuberosumVitis viniferaSpinacia oleraceaSorghum bicolorMedicago truncatulaTriticum aestivum.
The sequence of the synthetic peptide used for immunization is 81% homologues with the sequence in SnRK2.5 (AT5G63650).
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 18 μg total protein from Arabidopsis thaliana leaf.
Lane 2: 10 μg cytosolic protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.