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AT5G26751 ASK1 / Anti-ASK alpha antibody

Catalog Number: PHY0756S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT5G26751

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

ASKα (AT5G26751) is a stress-activated GSK3 protecting cell against oxidative stress. It has been shown that glucose-6-phosphate dehydrogenase 6 (AT5G40760) is the target of ASKa.

  Synonyms:

ASK1, ARABIDOPSIS GSK ALPHA, ARABIDOPSIS THALIANA SHAGGY-RELATED KINASE 11, ASKALPHA, ATSK11, SHAGGY-RELATED KINASE 11, SK 11

  Immunogen:

KLH-conjugated synthetic peptide of ASKα derived from Arabidopsis thaliana AT5G26751.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Applications: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

46 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 1 μg total protein from Arabidopsis thaliana leaf.
Lane 2: 5 μg total protein from Arabidopsis thaliana leaf.
Lane 3: 10 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.