RACK1 has been proved to regulate plant hormone signaling, stress responses and multiple processes during plant growth and development. It is a scaffold protein that acts downstream of and is phosphorylated by WNK8 in the glucose responsiveness and flowering pathways. In Arabidopsis, a total of three RACK proteins were found: AT1G18080 (RACK1A), AT1G48630 (RACK1B) and AT3G18130 (RACK1C).
RACK1A, ATARCA, ATRACK1, RACK1A_AT, RECEPTOR FOR ACTIVATED C KINASE 1 A, SAC53, SUPPRESSOR OF ACAULIS 53
KLH-conjugated synthetic peptide of RACK1A derived fromArabidopsis thalianaAT1G18080
Serum Peptide affinity form antibody available upon request at firstname.lastname@example.org.
Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
For more species homologues information, please contact tech support at email@example.com.
Lane 1: 10 μg total protein fromArabidopsis thalianaleaf. Lane 2: 25.16 μg total protein fromArabidopsis thalianaleaf. Lane 3: 50.32 μg total protein fromArabidopsis thalianaleaf. Electrophoresis:15% SDS-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:1000 dilution overnight at 4℃. Secondary antibody:1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.