ABI5 is a member of the basic leucine zipper transcription factor family, involved in ABA signalling during seed maturation and germination. The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes.
ABI5, ABA INSENSITIVE 5, ATABI5, DPBF1, GIA1, GROWTH-INSENSITIVITY TO ABA 1
KLH-conjugated synthetic peptide of ABI5 derived fromArabidopsis thalianaAT2G36270.
Serum Peptide affinity form antibody available upon request at email@example.com.
Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000), ChIP Note: Optimal dilutions/concentrations should be determined by the end user.
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99%% homologues with the sequence inBrassica napus. For more species homologues information, please contact tech support at firstname.lastname@example.org.
Lane 1: 10 μg total protein fromArabidopsis thalianaleaf. Lane 2: 25.16 μg total protein fromArabidopsis thalianaleaf. Lane 3: 50.32 μg total protein fromArabidopsis thalianaleaf. Electrophoresis:15% SDS-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:1000 dilution overnight at 4℃. Secondary antibody:1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.