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Catalog #79560 RIPK1 Kinase Assay Kit

The RIPK1 Kinase Assay Kit is designed to measure RIPK1 kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.
Description

The RIPK1 Kinase Assay Kit is designed to measure RIPK1 kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.

Materials Required But Not Supplied

ADP-Glo® Kinase Assay (Promega #V6930)

Dithiothreitol (DTT, 1 M; optional)

Microplate reader capable of reading luminescence

Adjustable micropipettor and sterile tips

30°C incubator

 
Applications
Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
 
Supplied As
The RIPK1 Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant RIPK1 enzyme, RIPK1 substrate, ATP and kinase assay buffer for 100 enzyme reactions.
 
Storage/Stability
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
 
Background
RIPK1 is a member of the receptor-interacting protein kinase (RIP) family. It plays an important role in cell survival as well as cell death including apoptosis and necroptosis. RIPK1 is highly expressed in Alzheimer’s disease (AD) brains. Importantly, it was shown that inhibition of RIPK1 results in decrease of amyloid and inflammation levels as well as accelerating amyloid beta peptide (Aβ) degradation by microglia in vitro.
 
Synonym(s)
RCK, MAP3K19, receptor-interacting serine/threonine-protein kinase 1 isoform 1, receptor-interacting serine/threonine-protein kinase 1, serine/threonine-protein kinase RIP, cell death protein RIP, receptor-interacting protein 1, receptor (TNFRSF)-interact
 
Format

Components

 

 
Instructions for Use
See assay kit data sheet for detailed protocol.
 
References
Ofengeim D., Proc. Natl. Acad. Sci. USA 114(41): E8788-E8797 (2017)
 
Warnings
Avoid freeze/thaw cycles.