AFH1 is a nonprocessive formin that moves from the barbered end to the side of an actin filament after the nucleation event. It might be involved in the organization and polarity of the actin cytoskeleton. And AFH1 is involved in polar pollen cell growth process by maintaining tip-focused cell membrane expansion for the polar extension of pollen tubes.
KLH-conjugated synthetic peptide of AFH1 derived fromArabidopsis thalianaAT3G25500.
Serum Peptide affinity form antibody available upon request at email@example.com.
Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence inZea mays, Sorghum bicolor. The sequence of the synthetic peptide used for immunization is 84% homologues with the sequence in ATFH6 (AT5G67470), AtFH2 (AT2G43800). For more species homologues information, please contact tech support at firstname.lastname@example.org.
Lane 1: 30 μg total protein fromArabidopsis thalianaleaf. Lane 2: 60 μg total protein fromArabidopsis thalianaleaf. Lane 3: 15 μg soluble protein fromArabidopsis thalianaleaf. Lane 4: 30 μg soluble protein fromArabidopsis thalianaleaf. Electrophoresis:10% SDS-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:1000 dilution overnight at 4℃. Secondary antibody:1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.