HOS1 is a novel protein with a RING finger motif near the amino terminus. HOS1 physically interacts with ICE1 and mediates the ubiquitination of ICE1 both in vitro and in vivo.
HOS1, EARLY IN SHORT DAYS 6, ESD6, HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 1
KLH-conjugated synthetic peptide of HOS1 derived fromArabidopsis thalianaAT2G39810.
Immunogen affinity purified
Reconstitution with 150µl of 0.01 M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
105 / 120 kDa
For more species homologues information, please contact tech support at firstname.lastname@example.org.
4 μg nuclear protein fromArabidopsis thaliana. Electrophoresis:10% SDS-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:1000 dilution overnight at 4℃. Secondary antibody:1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.