|The BisulFlash™ DNA Modification Kit is a complete set of optimized buffers and reagents to perform DNA modification using an expedited DNA bisulfite conversion technology developed by Epigentek, geared specifically for methylation-specific PCR (MSP) as well as post-bisulfite based NGS applications. Through a proprietary composition which allows DNA denaturation and bisulfite conversion to be processed at the same time, the complete procedure is reduced to only 30 minutes. Furthermore, it prevents more than 90% of DNA loss, completely converting unmethylated cytosine into uracil.
Perfecting Bisulfite Conversion
Traditional methods involve a separate denaturation step followed by a subsequent sodium bisulfite DNA conversion step -- but with the BisulFlash™ method, DNA denaturation status is concurrently sustained throughout the entire DNA bisulfite conversionprocess. This breakthrough approach enables the DNA conversion process to be significantly faster with higher conversion efficiency and accuracy. We continue to innovate with the development of the new BisulFlash™ kit by identifying four critical components of bisulfite conversion:
The convenient ready-to-use, DNA conversion mix solution and single temperature incubation along with the features mentioned above allow for true perfection in bisulfite conversion. The BisulFlash™ DNA Modification Kit is suitable for MS-PCR/MSP and real time MSP.
Bisulfite Sequencing Option
Fig. 1. High accuracy of DNA conversion is achieved by the BisulFlash™ DNA Modification Kit. 50 ng of genomic DNA methylated in all CpG sites by DNA methylases was treated with the BisulFlash™ DNA Modification Kit. Converted DNA was amplified by real time qPCR using primers for multiple promoters containing numerous CpG sites and then directly sequenced.
Comparative Overview of Commercial Kits