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快速高尔基染色试剂盒(神经元和胶质细胞)

FD Rapid GolgiStain™ Kit (large)
PK401
常备现货

快速高尔基染色试剂盒是美国FD NeuroTechnologies, Inc.公司生产的,用于神经元和胶质细胞形态学研究的完整Golgi-Cox染色体系,FD快速高尔基染色试剂盒货号PK401,快速高尔基染色试剂盒仅用于体外研究,快速高尔基染色试剂盒不能用于诊断或其它用途。

 

Golgi-Cox浸染法是研究神经元和胶质细胞正常和非正常形态最有效的方法之一。使用Golgi技术,在药物处理过的动物脑中和因神经疾病死亡的病人脑中发现了神经树突和树突棘微小的形态改变。然而Golgi染色法不可靠且费时,成为这种方法广泛应用的障碍。

 

美国FD NeuroTechnologies, Inc.公司生产的FD Rapid GolgiStainTM Kit(FD快速Golgi染色试剂盒) 是根据Ramon-Moliner, Glaser2和Van der Loos5所阐述的方法的原则设计的。这个试剂盒不仅极大改进并简化了Golgi-Cox技术,而且被证实在显示神经元和胶质细胞,特别是树突棘的形态细节上极为灵敏可靠。FD Rapid GolgiStainTM Kit已被广泛测试并用于数种动物脑及去世病人的脑。

 

试剂盒染色图片举例:

     

 

试剂盒组成:

室温下保存

溶液A 125 ml

溶液B 125 ml

溶液C 125 ml x 2

溶液D 125 ml

溶液E 125 ml

玻璃样品回收器 2

天然毛画笔 3

滴瓶 1

用户指南 1

 

需要但未包括在试剂盒中的物品:

双蒸水或去离子水

塑料管或玻璃管或小瓶

组织学用品和设备,包括明胶包被的显微镜载玻片,盖玻片,染色瓶,乙醇,二甲苯或其替代品,树脂封固剂(如Permount®),及光镜。

 

参考文献:


1.Corsi P. (1987) Camillo Golgi’s morphological approach to neuroanatomy. In Masland RL, Portera-Sanchez A and Toffano G (eds.), Neuroplasticity: a new therapeutic tool in the CNS pathology, pp 1-7. Berlin: Springer.
2.Ramón-Moliner E. (1970) The Golgi-Cox technique. In Nauta WJH and Ebbesson SOE (eds.), Contemporary Methods in Neuroanatomy. pp 32-55, New York: Springer.
3.Graveland GA, Williams RS, and DiFiglia M. (1985) Evidence for degenerative and regenerative changes in neostriatal spiny neurons in Huntington’s disease. Science. 227:770-3.
4.Robinson TE, and Kolb B. (1997) Persistent structural modification in nucleus accumbens and prefrontal cortex neurons produced by previous experience with amphetamine. J. Neurosci. 17:8491-7.
5.Glaser ME, and Van der Loos H. (1981) Analysis of thick brain sections by obverse-reverse computer microscopy: application of a new, high clarity Golgi-Nissl stain. J. Neurosci. Meth. 4:117-25.

 

 

Golgi-Cox impregnation1, 2 has been one of the most effective techniques for studying both the normal and abnormal morphology of neurons as well as glia. Using the Golgi technique, subtle morphological alterations in neuronal dendrites and dendritic spines have been discovered in the brains of animals treated with drugs as well as in the postmortem brains of patients with neurological diseases3, 4. However, the unreliability and the time-consuming process of Golgi staining have been major obstacles to the widespread application of this technique.

FD Rapid GolgiStain™ Kit is designed based on the principle of the methods described by Ramón- Moliner2, Glaser and Van der Loos5. This kit has not only dramatically improved and simplified the Golgi-Cox technique but has also proven to be extremely reliable and sensitive for demonstrating morphological details of neurons and glia, especially dendritic spines. The FD Rapid GolgiStain™ Kit has been tested extensively and widely used on the brains from several species of animals as well as on the specimens of postmortem human brains.


 

Kit contents:

Store at room temperature

Solution A                                         250 ml
Solution B                                         250 ml 
Solution C                                         250 ml x 2 
Solution D                                         250 ml 
Solution E                                         250 ml 
Glass Specimen Retriever                    2         
Natural hair paintbrush                         3       
Dropping bottle                                     1       
User Manual                                         1  

Materials required but not included:

  • Double distilled or deionized water.
  • Plastic or glass tubes or vials. 
  • Histological supplies and equipment, including gelatin-coated microscope slides, coverslips, staining jars, ethanol,  xylene or xylene substitutes, resinous mounting medium (e.g. Permount®), and a light microscope.

References:

  1. Corsi P. (1987) Camillo Golgi’s morphological approach to neuroanatomy. In Masland RL, Portera-Sanchez A and Toffano G (eds.), Neuroplasticity: a new therapeutic tool in the CNS pathology, pp 1-7. Berlin: Springer.
  2. Ramón-Moliner E. (1970) The Golgi-Cox technique. In Nauta WJH and Ebbesson SOE (eds.), Contemporary Methods in Neuroanatomy. pp 32-55, New York: Springer.
  3. Graveland GA, Williams RS, and DiFiglia M. (1985) Evidence for degenerative and regenerative changes in neostriatal spiny neurons in Huntington’s disease. Science. 227:770-3.
  4. Robinson TE, and Kolb B. (1997) Persistent structural modification in nucleus accumbens and prefrontal cortex neurons produced by previous experience with amphetamine. J. Neurosci. 17:8491-7.
  5. Glaser ME, and Van der Loos H. (1981) Analysis of thick brain sections by obverse-reverse computer microscopy: application of a new, high clarity Golgi-Nissl stain. J. Neurosci. 

 

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