Reconstitution with 150µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
This antibody specifically recognizes the 6X His-Tag at C-terminal or N-terminal of recombinant proteins, which can be used to detect His-Tag fusion protein of various expression vector.
Lane 1: 0.5 ng His-tagged fusion protein. Lane 2: 1 ng His-tagged fusion protein. Lane 3: 2.5 ng His-tagged fusion protein. Lane 4: 5 ng His-tagged fusion protein. Electrophoresis:12% SDS-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1 h. Blocking:5% skim milk at RT or 4℃ for 1 h. Primary antibody:1:1000 dilution overnight at 4℃. Secondary antibody:1:5000 dilution using Goat Anti-Mouse IgG H&L (HRP) (Cat# PHY6006). Detection:using chemiluminescence substrate and image were captured with CCD camera.