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His / Anti-His tag antibody PHY5010

His / Anti-His tag antibody
Catalog Number: PHY5010
Size: 150 µg

 

Product Information

  Description:

Mouse monoclonal antibody

  Synonyms:

His

  Immunogen:

Synthetic peptide of fragment (HHHHHH) His Tag.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Confirmed Reactivity: 

This antibody specifically recognizes the 6X His-Tag at C-terminal or N-terminal of recombinant proteins, which can be used to detect His-Tag fusion protein of various expression vector.

 

Application Example

Lane 1: 0.5 ng His-tagged fusion protein. 
Lane 2: 1 ng His-tagged fusion protein. 
Lane 3: 2.5 ng His-tagged fusion protein. 
Lane 4: 5 ng His-tagged fusion protein.
Electrophoresis: 12% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:5000 dilution using Goat Anti-Mouse IgG H&L (HRP) (Cat# PHY6006). 
Detection: using chemiluminescence substrate and image were captured with CCD camera.