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PsbD / Anti-D2 subunit of Photosystem II, C-terminal antibody PHY0060

PsbD / Anti-D2 subunit of Photosystem II, C-terminal antibody
Catalog Number: PHY0060
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana, Hordeum vulgare subsp. Vulgare, Zea mays, Oryza sativa Indica Group, Glycine max.
Database Access Number: ATCG00270

 

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

The D1/D2 (PsbA/PsbD) reaction center heterodimer binds P680, the primary electron donor of PSII as well as several subsequent electron acceptors. D2 is needed for assembly of a stable PSII complex.

  Synonyms:

PsbD

  Immunogen:

KLH-conjugated synthetic peptide derived from C-terminal of D2 subunit in Arabidopsis thaliana ATCG00270.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

39.5 / 33 kDa (Arabidopsis thaliana)

  Confirmed Reactivity: 

Arabidopsis thaliana, Hordeum vulgare subsp. Vulgare, Zea mays, Oryza sativa Indica Group, Glycine max.

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Solanum tuberosum, Cucumis sativus, Sorghum bicolor, Physcomitrella patens, Triticum aestivum, Nicotiana tabacum, Setaria viridis, Spinacia oleracea, Populus trichocarpa, Vitis vinifera, Chlamydomonas reinhardtii, Medicago truncatula.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

 Example 1

1-3 is thylakiod membrane protein from Arabidopsis thaliana leaf containing 0.1μg, 0.25μg, and 0.5μg of chlorophyll, respectively.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.