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PsbB / Anti-CP47 subunit of Photosystem II antibody PHY0058A

PsbB / Anti-CP47 subunit of Photosystem II antibody
Catalog Number: PHY0058A
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana, Glycine max, Zea mays, Oryza sativa Indica Group, Hordeum vulgare subsp. Vulgare
Database Access Number: ATCG00680

 

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

CP47 is one of the components of the core complex of photosystem II (PSII). It binds chlorophyll and helps catalyze the primary light-induced photochemical processes of PSII. CP47 is encoded by ATCG00680.

  Synonyms:

PsbB

  Immunogen:

KLH-conjugated synthetic peptide of CP47 subunit derived from Arabidopsis thaliana ATCG00680.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

56 / 47 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana, Glycine max, Zea mays, Oryza sativa Indica Group, Hordeum vulgare subsp. Vulgare

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Physcomitrella patens, Spinacia oleracea, Medicago truncatula, Gossypium raimondii, Spinacia oleracea, Brassica napus, Nicotiana tabacum, Setaria viridis, Populus trichocarpa, Vitis vinifera, Cucumis sativus, Sorghum bicolor, Nicotiana tabacum, and 80-99% homologues with the sequence in Triticum aestivum, Chlamydomonas reinhardtii.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

1-3 is thylakiod membrane protein from Arabidopsis thaliana leaf containing 0.1μg, 0.25μg, and 0.5μg of chlorophyll, respectively.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.