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PsbC / Anti-CP43 subunit of Photosystem II, E-loop antibody PHY0059A

PsbC / Anti-CP43 subunit of Photosystem II, E-loop antibody
Catalog Number: PHY0059A
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: ATCG00280

 

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

CP43 is a component of the proximal antenna of PSII and contains six transmembrane alpha helices and five hydrophilic loops that connect the membrane-spanning domains. CP43 is encoded by the psbC gene (ATCG00280).

  Synonyms:

PsbC

  Immunogen:

KLH-conjugated synthetic peptide derived from E-loop of PsbC in Arabidopsis thaliana ATCG00280.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

51.8 / 40 kDa (Arabidopsis thaliana)

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Gossypium raimondii, Solanum lycopersicum, Populus trichocarpa, Vitis vinifera, Solanum tuberosum, Solanum lycopersicum, Nicotiana tabacum, Cucumis sativus, Setaria viridis, Triticum aestivum, Oryza sativa Japonica Group, Sorghum bicolor, and 80-99% homologues with the sequence in Physcomitrella patens, Glycine max, Spinacia oleracea, Medicago truncatula f. tricycle, Chlamydomonas reinhardtii.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

 Example 1

1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.1 μg, 0.25 μg, and 0.5 μg of chlorophyll, respectively. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.