PsbP is a 23 kD extrinsic subunit of the oxygen evolving complex of Photosystem II. PsbP participates in the regulation of oxygen evolution and stabilizes the oxygen evolving complex of PSII.
PsbP, 23 kDa protein of the oxygen evolving complex (OEC) of PSII, Oxygen-evolving enhancer protein 2-1, chloroplastic
KLH-conjugated synthetic peptide of PsbP derived fromArabidopsis thalianaAT1G06680.
Serum Peptide affinity form antibody available upon request at email@example.com.
Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence inNicotiana tabacum, Solanum lycopersicum,and 80-99% homologues with the sequence inMedicago truncatula, Cucumis sativus. For more species homologues information, please contact tech support at firstname.lastname@example.org.
1-2 is thylakoid membrane protein from WT ofArabidopsis thalianaleaf containing 0.25 μg and 0.5 μg of chlorophyll, respectively. 3 is thylakoid membrane protein from PSII mutant ofArabidopsis thalianaleaf containing 0.5 μg of chlorophyll. Electrophoresis:15% SDS-Urea-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:2000 dilution overnight at 4℃. Secondary antibody:1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.