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PsbQ / Anti-PsbQ subunit of oxygen evolving complex of Photosystem II antibody PHY0081S

PsbQ / Anti-PsbQ subunit of oxygen evolving complex of Photosystem II antibody
Catalog Number: PHY0081S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana 
Database Access Number: AT4G21280
 

 

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PsbQ is a 16 kD extrinsic subunit of the oxygen evolving complex of Photosystem II. PsbQ participates in the regulation of oxygen evolution and stabilizes the oxygen evolving complex of PSII. It is required for photoautotrophic growth under conditions of low light.

  Synonyms:

PsbQ, OEC 16 kDa subunit, OEE3, PSBQA,16 kDa subunit of oxygen evolving system of photosystem II, Oxygen-evolving enhancer protein 3-1, chloroplastic

  Immunogen:

KLH-conjugated synthetic peptide of PsbQ derived from Arabidopsis thaliana AT4G21280.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

24 / 16 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

1-2 is thylakoid membrane protein from WT of Arabidopsis thaliana leaf containing 1.25 μg and 2.5 μg of chlorophyll, respectively. 
3 is thylakoid membrane protein from Arabidopsis mutant with low accumulation of PSII containing 2.5 μg of chlorophyll. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.