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Lhcb1 / Anti-Lhcb1 protein of LHCII antibody PHY0487S

Lhcb1 / Anti-Lhcb1 protein of LHCII antibody
Catalog Number: PHY0487S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT1G29910
 

 

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

The light-harvesting complex (LHC) functions as a light receptor; it captures and delivers excitation energy to photosystem. Lhcb1, Lhcb2 and Lhcb3 are the major pigment-binding proteins which are encoded by Lhcb1, Lhcb2 and Lhcb3 genes, respectively. Lhcb1 and Lhcb2 are the most abundant proteins in the light harvesting antenna complex.

  Synonyms:

Lhcb1

  Immunogen:

KLH-conjugated synthetic peptide of Lhcb1 derived from Arabidopsis thaliana AT1G29910.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:5000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

28 / 30 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.025 μg, 0.05 μg, and 0.1 μg of chlorophyll, respectively. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.