The light-harvesting complex (LHC) functions as a light receptor; it captures and delivers excitation energy to photosystem. Lhcb1, Lhcb2 and Lhcb3 are the major pigment-binding proteins which are encoded by Lhcb1, Lhcb2 and Lhcb3 genes, respectively. Lhcb1 and Lhcb2 are the most abundant proteins in the light harvesting antenna complex.
KLH-conjugated synthetic peptide of Lhcb1 derived fromArabidopsis thalianaAT1G29910.
Serum Peptide affinity form antibody available upon request at firstname.lastname@example.org.
Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:5000) Note: Optimal dilutions/concentrations should be determined by the end user.
28 / 30 kDa
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence inBrassica napus. For more species homologues information, please contact tech support at email@example.com.
1-3 is thylakoid membrane protein fromArabidopsis thalianaleaf containing 0.025 μg, 0.05 μg, and 0.1 μg of chlorophyll, respectively. Electrophoresis:15% SDS-Urea-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:5000 dilution overnight at 4℃. Secondary antibody:1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.