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SYBR Green I荧光染料(电泳)10000x

dsGreen Gel Staining Solution, 10000×
20010   1ml

SYBR Green I is a sensitive dsDNA binding dye, which can be used for routine DNA detection in agarose and polyacrylamide gels.

Unlike Ethidium, SYBR Green I is highly selective towards double stranded DNA, much less harmful, and offer better sensitivity.

A comparison between Ethidium Bromide and SYBR Green I

Feature Ethidium Bromide SYBR Green I
Fluorescence Red (615 nm) Green (524 nm)
Excitation maximum 302 nm 454 nm
Excitation light source UV only Blue light or UV
Sensitivity 2 ng / band(dsDNA)
100 ng / band (RNA)
0.08 ng / band (dsDNA)
1-2 ng / band (oligonucleotides)
Health hazard High Low

 

General properties

Appearance: orange solution
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download

Spectral properties

Excitation maximum, nm: 454
Extinction coefficient at excitation maximum, Lmol-1cm-1: 73000
Emission maximum, nm: 524
Fluorescence quantum yield: 0.8