PsbZ is a photosystem II subunit with a molecular mass of 6.541 kDa. It has been shown that PsbZ is essential in the interaction between PS II and the light harvesting complex II.
KLH-conjugated synthetic peptide of PsbZ derived fromArabidopsis thalianaATCG00300.
Immunogen affinity purified
Reconstitution with 150µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence inNicotiana tabacum, Populus trichocarpa, Medicago truncatula, and 80-99% homologues with the sequence inSpinacia oleracea, Vitis vinifera, Triticum aestivum, Oryza sativa Japonica Group, Zea mays, Panicum virgatum, Cucumis sativus, Physcomitrella patens, Glycine max, Gossypium raimondii. For more species homologues information, please contact tech support at firstname.lastname@example.org.
1-3 is thylakoid membrane protein from WT ofArabidopsis thalianaleaf containing 0.01 μg, 0.025 μg, and 0.05 μg of chlorophyll, respectively. 4 is thylakoid membrane protein from PSII mutant ofArabidopsis thalianaleaf containing 0.05 μg of chlorophyll. Electrophoresis:Tricine-SDS-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:2000 dilution overnight at 4℃. Secondary antibody:1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.