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PsbS / Anti-PsbS protein antibody PHY0345

PsbS / Anti-PsbS protein antibody
Catalog Number: PHY0345
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT1G44575

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PsbS is a ubiquitous pigment-binding protein associated with photosystem II (PSII) of higher plants, which is involved in nonphotochemical quenching rather than in photosynthesis. Mutant has a normal violaxanthin cycle but has a limited capacity of quenching singlet excited chlorophylls and is tolerant to lipid peroxidation.

  Synonyms:

PsbS, 22 kDa Lhc-like PSII protein

  Immunogen:

KLH-conjugated synthetic peptide derived of PsbS from Arabidopsis thaliana AT1G44575.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

28 / 21 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Brassica rapa, and 80-99% homologues with the sequence in Gossypium raimondii, Vitis vinifera, Glycine max, Populus trichocarpa, Physcomitrella patens, Solanum tuberosum, Nicotiana tabacum, Cucumis sativus.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

 Example 1

1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.1 μg, 0.25 μg, and 0.5 μg of chlorophyll, respectively.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.